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Equipment

Leica TCS SP2 Confocal Microscope

Leica TCS SP2 Confocal Microscope Leica TCS SP2 Confocal Microscope

It has six laser lines in the visible spectrum (458, 476, 488, 514, 561 and 633 nm) and two lines in the ultraviolet spectrum (351, 364 nm), three internal adjustable bandwidth and wavelength detection channels and an external transmitted light detector for clear field images.

Available lenses: 5X/0.15, 10X/0.4, 20X/0.7 OIL, 40X/1.25-0.75 OIL, 63X/1.4-0.6 OIL, 100X1.4-0.7 OIL.
Motorized stage, with the possibility of thermostatized chamber assembly with CO2 for in vivo experiments.
Possibility of: electronic zoom, rotation of the scanning beam.
Acquisition of multidimensional images up to 8D: x, y, z, t, α,λ, i, d (image area, height, time, rotation, wavelength, intensity, xy location and tile scan).
Spectral studies on emission calculation of an unknown fluorochrome or autofluorescence, elimination of the crossing between fluorochromes with overlapping emission.

Laboratory using this equipment

Microscopy Unit
Servicio Interdepartamental de Investigación
UNE-EN ISO 9001
Link to REMOA web page. External link Open a new window.
Confocal Microscopy Laboratory in the Red de Laboratorios of the Comunidad de Madrid. External link Open a new window.
School of Medicine - Universidad Autónoma de Madrid. External link Open a new window.
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